Gardel Lab Microscopes
Multi-spectral Spinning Disk Confocal Microscope with Mosaic FRAP
Specifications and Manuals
• Yokogawa CSU-X1 (2 camera port option) confocal scanhead (manual)
• Nikon Eclipse Ti-PFS inverted microscope (manual)
• Photonic Instruments Mosaic micro-mirror array for targeted illumination/FRAP (405 nm)
• Spectral Applied Research Laser Merge Module (491 nm, 561 nm, and 643 nm Lasers) (manual)
• Molecular Devices MetaMorph software
• Vibration-isolation table (Thor Labs)
| Reverse Dichroics in CSUX head: | ||||
| Inverse dichroic for 405/488/568/647 | Semrock | DI01-T405/488/568/647-13X15X0.5 | ||
| Emission Filters in CSUX: | Em. low/high pass (nm) | |||
| Emission Filter 525/50M | 500/550 | Chroma | ET525/50M | |
| Emission Filter 605/70M | 570/640 | Chroma | ET605/70M | |
| Emission Filter 700/75M | 664/737 | Chroma | ET700/75M | |
| Low-pass filter (500 nm) | ||||
| Polarization filter | ||||
| Cubes in Nikon Turret: | Ex. | Em. | ||
| Texas Red | 532/582 | 604/644 | Semrock | TXRed-4040B-NTE |
| GFP | 457/487 | 502/538 | Semrock | GFP-3035B-NTE |
| Epi-block consisting of blank cube |
Multimode High Resolution Epi-fluorescence Microscope for Micromanipulation
Specifications and Manuals
• Nikon Eclipse Ti-PFS inverted microscope (manual)
• Nikon White Light TIRF
• Motorized Micromanipulator (Sutter MP-285) (manual)
(reference manual)
• Xenoworks Microinjector
• NevTek air stream stage incubator
• Vibration-isolation table (Thor Labs)
Multi-Spectral Spinning Disk Confocal Microscope With TIRF Adaptor
Specifications and Manuals
• Nikon Eclipse Ti-PFS inverted microscope with motorized TIRF adaptor(manual)
• Yokogawa CSU-X1 (2 camera port option) confocal scanhead (manual)
• Photometrics Evolve EMCCD camera
• Spectral Applied Research Laser Merge Module (491 nm, 561 nm, and 643 nm Lasers) (manual)
• NevTek air stream stage incubator
• Vibration-isolation table (Thor Labs)
| Reverse Dichroics in CSUX head: | |||
| Inverse dichroic for 405/488/568/647 | Semrock | DI01-T405/488/568/647-13X15X0.5 | |
| Cubes in Nikon Turret: | |||
| 405/488/561/640 quad filter cube for TIRF | Chroma | TRF89901 | |
| Filters in CSU-X | Em. Low/High Pass (nm) | ||
| Emission Filter 525/50M | 500/550 | Chroma | ET525/50M |
| Emission Filter 605/70M | 570/640 | Chroma | ET605/70M |
| Emission Filter 700/75M | 664/737 | Chroma | ET700/75M |
| Low-pass filter (500 nm) | |||
| Polarization filter |
Additional Imaging Equipment
Microscope Objectives
| Objective | Immersion | Numerical Aperture | Working Distance (mm) | DIC/Phase | Correction Collar |
| 4x Plan Fluor | Air | 0.13 | 16.4 | PhL | No |
| 10x Plan Fluor | Air | 0.3 | 16 | Ph1 | No |
| 20x Plan Fluor ELWD | Air | 0.45 | 7.4 | Ph1 | Yes |
| 20x Plan Fluor | Oil/Water | 0.75 | 0.35 | DIC N2 | Yes |
| 20x Plan Fluor | Oil/Water | 0.75 | 0.35 | DIC N2 | Yes |
| 40x Plan Fluor | Oil | 1.3 | 0.2 | DIC H/N2 | No |
| 40x Plan Apo | Oil | 1 | 0.16 | Ph3 | No |
| 40x Apo LWD λS | Water | 1.15 | 0.59-0.61 | DIC N2 | Yes |
| 60x Plan Apo | Water | 1.2 | 0.28-0.31 | DIC N2 | Yes |
| 60x Plan Apo | Water | 1.2 | 0.27 | DIC N2 | Yes |
| 60x Apo TIRF | Oil | 1.49 | 0.12 | DIC N2 | Yes |
| 100x Plan Apo | Oil | 1.4 | 0.13 | Ph3 | No |
| 100x Apo TIRF | Oil | 1.49 | 0.12 | DIC N2 | Yes |
Other Portable Imaging Equipment
• Two Ph1 condenser rings for phase contrast imaging
• Two Ph3 condenser rings for phase contrast imaging
• N2 condenser Nomarski prism
• 60xII turret Nomarski prism
• Chamlide FC-5N Air/CO2 mixer (for use with stage incubator) (Manual)
• PhL condenser ring for phase contrast imaging
• NR condenser Nomarski prism
• 100xI turret Nomarski prism
• Chamlide TC stagetop incubator system (Manual)