Gardel Lab Microscopes

 

Multi-spectral Spinning Disk Confocal Microscope with Mosaic FRAP

confocal

Specifications and Manuals

• Yokogawa CSU-X1 (2 camera port option) confocal scanhead (manual) • Lumen 200- 200 Watt Metal halide light source (Prior) (manual)
• Nikon Eclipse Ti-PFS inverted microscope (manual) • Proscan II XY linear encoded motarized stage (Prior) (manual)
• Photonic Instruments Mosaic micro-mirror array for targeted illumination/FRAP (405 nm) • Uniblitz Shutter (transmitted light)
• Spectral Applied Research Laser Merge Module (491 nm, 561 nm, and 643 nm Lasers) (manual) • NevTek air stream stage incubator
• Molecular Devices MetaMorph software • Photometrics Coolsnap HQ2 CCD camera (manual) (brochure)
• Vibration-isolation table (Thor Labs) • Andor Zyla sCMOS camera

 

Reverse Dichroics in CSUX head:        
Inverse dichroic for 405/488/568/647  SemrockDI01-T405/488/568/647-13X15X0.5
Emission Filters in CSUX:Em. low/high pass (nm)   
Emission Filter 525/50M500/550 ChromaET525/50M
Emission Filter 605/70M570/640 ChromaET605/70M
Emission Filter 700/75M664/737  ChromaET700/75M
Low-pass filter (500 nm)    
Polarization filter    
Cubes in Nikon Turret:Ex.Em.  
Texas Red532/582604/644SemrockTXRed-4040B-NTE
GFP457/487502/538SemrockGFP-3035B-NTE
Epi-block consisting of blank cube    

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Multimode High Resolution Epi-fluorescence Microscope for Micromanipulation

widefield

Specifications and Manuals

• Nikon Eclipse Ti-PFS inverted microscope (manual) • Photometrics Coolsnap HQ2 CCD camera (manual)
• Nikon White Light TIRF • Lumen Pro-200 Watt Metal halide light source (Prior) (manual)
• Motorized Micromanipulator (Sutter MP-285) (manual)
(reference manual)
• Proscan II XY linear encoded motarized stage (Prior) (manual)
• Xenoworks Microinjector • Prior Transmitted Shutter
• NevTek air stream stage incubator • Molecular Devices MetaMorph software
• Vibration-isolation table (Thor Labs) • Uniblitz Shutter (transmitted light)

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Multi-Spectral Spinning Disk Confocal Microscope With TIRF Adaptor

Specifications and Manuals

• Nikon Eclipse Ti-PFS inverted microscope with motorized TIRF adaptor(manual) • Lumen 200- 200 Watt Metal halide light source (Prior) (manual)
• Yokogawa CSU-X1 (2 camera port option) confocal scanhead (manual) • Photometrics Coolsnap HQ2 CCD camera (manual) (brochure)
• Photometrics Evolve EMCCD camera • Uniblitz Shutter (transmitted light)
• Spectral Applied Research Laser Merge Module (491 nm, 561 nm, and 643 nm Lasers) (manual) • Proscan III XY linear encoded motarized stage (Prior) (manual)
• NevTek air stream stage incubator • Lumen 200- 200 Watt Metal halide light source (Prior) (manual)
• Vibration-isolation table (Thor Labs) • Molecular Devices MetaMorph software

 

Reverse Dichroics in CSUX head:      
Inverse dichroic for 405/488/568/647 SemrockDI01-T405/488/568/647-13X15X0.5
Cubes in Nikon Turret:   
405/488/561/640 quad filter cube for TIRF ChromaTRF89901
Filters in CSU-XEm. Low/High Pass (nm)  
Emission Filter 525/50M500/550 ChromaET525/50M
Emission Filter 605/70M570/640ChromaET605/70M
Emission Filter 700/75M664/737 ChromaET700/75M
Low-pass filter (500 nm)   
Polarization filter   

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Additional Imaging Equipment

Microscope Objectives

Objective Immersion Numerical Aperture Working Distance (mm) DIC/Phase Correction Collar
4x Plan FluorAir0.1316.4PhLNo
10x Plan FluorAir0.316Ph1No
20x Plan Fluor ELWDAir0.457.4Ph1Yes
20x Plan FluorOil/Water0.750.35DIC N2Yes
20x Plan FluorOil/Water0.750.35DIC N2Yes
40x Plan FluorOil1.30.2DIC H/N2No
40x Plan ApoOil10.16Ph3No
40x Apo LWD λSWater1.150.59-0.61DIC N2Yes
60x Plan ApoWater1.20.28-0.31DIC N2Yes
60x Plan ApoWater1.20.27DIC N2Yes
60x Apo TIRFOil1.490.12DIC N2Yes
100x Plan ApoOil1.40.13Ph3No
100x Apo TIRFOil1.490.12DIC N2Yes

 

Other Portable Imaging Equipment

•Two Ph1 condenser rings for phase contrast imaging •PhL condenser ring for phase contrast imaging
•Two Ph3 condenser rings for phase contrast imaging •NR condenser Nomarski prism
•N2 condenser Nomarski prism •100xI turret Nomarski prism
•60xII turret Nomarski prism •Chamlide TC stagetop incubator system (Manual)
•Chamlide FC-5N Air/CO2 mixer (for use with stage incubator) (Manual)  

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